Porcine hepatocyte isolation and reversible immortalization mediated by retroviral transfer and site-specific recombination

AIM: To develop a hepatocyte cell line, we immortalized primary porcine hepatocytes with a retroviral vector SSR#69 containing the Simian Virus 40 T antigen (SV40Tag). METHODS: We first established a method of porcine hepatocyte isolation with a modified four-step retrograde perfusion technique. The...

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Main Authors: Meng, Fan-Ying, Chen, Zhi-Shui, Han, Meng, Hu, Xin-Peng, He, Xing-Xing, Liu, Yong, He, Wen-Tao, Huang, Wei, Guo, Hui, Zhou, Ping
Formato: Artigo
Idioma:Inglês
Publicado em: The WJG Press and Baishideng 2010
Assuntos:
Brief Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2848376/
https://ncbi.nlm.nih.gov/pubmed/20355246
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3748/wjg.v16.i13.1660
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