Expressed Alu repeats as a novel, reliable tool for normalization of real-time quantitative RT-PCR data

We describe a novel strategy for mRNA normalization in quantitative real-time PCR that is based on expressed Alu repeat amplification as a measure for the mRNA fraction. We show that expressed Alu repeat amplification is a fast, accurate normalization tool that can be successfully used for quantific...

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Detalhes bibliográficos
Main Authors: Marullo, Manuela, Zuccato, Chiara, Mariotti, Caterina, Lahiri, Nayana, Tabrizi, Sarah J, Di Donato, Stefano, Cattaneo, Elena
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2010
Assuntos:
Method
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2847721/
https://ncbi.nlm.nih.gov/pubmed/20109193
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/gb-2010-11-1-r9
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