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Quantification of Green Fluorescent Protein in Cellular Supernatant by Capillary Electrophoresis with Laser-Induced Fluorescence Detection for Measurement of Cell Death

A common method for quantifying cell death is measuring the concentration of lactate dehydrogenase (LDH) released by cells as their membranes become unstable. In cells expressing green fluorescent protein (GFP), degradation of the cell membrane also results in the release of GFP into the surrounding...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Swearingen, Kristian E., Loomis, Wendy P., Kehimkar, Benjamin, Cookson, Brad T., Dovichi, Norman J.
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2010
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2842605/
https://ncbi.nlm.nih.gov/pubmed/20298877
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.talanta.2010.01.041
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