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Multiplexed immunophenotyping of human antigen-presenting cells in whole blood by polychromatic flow cytometry
We describe two modular protocols for immunostaining and multiparameter flow cytometric analysis of major human antigen-presenting cells (dendritic cells, monocytes, B lymphocytes) in minimally manipulated whole blood. Simultaneous detection of up to eight colors is enabled by careful selection and...
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| Hauptverfasser: | , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
2010
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2839283/ https://ncbi.nlm.nih.gov/pubmed/20134434 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2009.246 |
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