A real-time RT-PCR for detection of clade 1 and 2 H5N1 Influenza A virus using Locked Nucleic Acid (LNA) TaqMan probes

BACKGROUND: The emergence and co-circulation of two different clades (clade 1 and 2) of H5N1 influenza viruses in Vietnam necessitates the availability of a diagnostic assay that can detect both variants. RESULTS: We developed a single real-time RT-PCR assay for detection of both clades of H5N1 viru...

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Bibliographic Details
Main Authors:	Tran Tan, Thanh, Pawestri, Hana Apsari, My, Ngoc Nghiem, Minh, Hien Vo, Syahrial, Harun, Vu, Trung Nguyen, van Doorn, H Rogier, Wertheim, Heiman FL, Van Vinh, Chau Nguyen, Quang, Ha Do, Farrar, Jeremy J, Tinh, Hien Tran, Sedyaningsih, Endang R, de Jong, Menno D
Format:	Artigo
Language:	Inglês
Published:	BioMed Central 2010
Subjects:	Methodology
Online Access:	https://ncbi.nlm.nih.gov/pmc/articles/PMC2838857/ https://ncbi.nlm.nih.gov/pubmed/20170549 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1743-422X-7-46
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A real-time RT-PCR for detection of clade 1 and 2 H5N1 Influenza A virus using Locked Nucleic Acid (LNA) TaqMan probes

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