Protein Crystallography Reveals a Role for the FS0 Cluster of Escherichia coli Nitrate Reductase A (NarGHI) in Enzyme Maturation

We have used site-directed mutagenesis, EPR spectroscopy, redox potentiometry, and protein crystallography to monitor assembly of the FS0 [4Fe-4S] cluster and molybdo-bis(pyranopterin guanine dinucleotide) cofactor (Mo-bisPGD) of the Escherichia coli nitrate reductase A (NarGHI) catalytic subunit (N...

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Detalhes bibliográficos
Main Authors: Rothery, Richard A., Bertero, Michela G., Spreter, Thomas, Bouromand, Nasim, Strynadka, Natalie C. J., Weiner, Joel H.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Biochemistry and Molecular Biology 2010
Assuntos:
Bioenergetics
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2838302/
https://ncbi.nlm.nih.gov/pubmed/20053990
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M109.066027
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