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Rapid De-O-glycosylation Concomitant with Peptide Labeling Using Microwave Radiation and an Alkyl Amine Base
Procedures are detailed for a quantitative release of O-linked glycans from peptides that now provide a shorter reaction time, a possible identification of O-linked sites, and a quantification of all reaction products. The release was initiated by a mild base, dimethylamine, and accelerated by micro...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2837759/ https://ncbi.nlm.nih.gov/pubmed/20178317 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ac902734w |
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