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Fluid Phase Endocytosis Contributes to Transfection of DNA by PEI-25

The understanding of internalization pathways of lipo- or polyplexes is crucial for engineering successful reagents for nonviral gene transfection. A known inhibitor of fluid phase endocytosis (FPE), rottlerin, was used to quantify the contribution of this pathway by flow cytometric and fluorescence...

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Detalhes bibliográficos
Main Authors: Hufnagel, Hansjörg, Hakim, Parvez, Lima, Aline, Hollfelder, Florian
Formato: Artigo
Idioma:Inglês
Publicado em: Nature Publishing Group 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2835228/
https://ncbi.nlm.nih.gov/pubmed/19532143
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/mt.2009.121
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