Propagation of human embryonic and induced pluripotent stem cells in an indirect co-culture system

We have developed and validated a microporous poly(ethylene terephthalate) membrane-based indirect co-culture system for human pluripotent stem cell (hPSC) propagation, which allows real-time conditioning of the culture medium with human fibroblasts while maintaining the complete separation of the t...

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Main Authors: Abraham, Sheena, Sheridan, Steven D., Laurent, Louise C., Albert, Kelsey, Stubban, Christopher, Ulitsky, Igor, Miller, Bradley, Loring, Jeanne F., Rao, Raj R.
Format: Artigo
Jezik:Inglês
Izdano: 2010
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Article
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2834855/
https://ncbi.nlm.nih.gov/pubmed/20117095
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrc.2010.01.101
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