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A versatile polyacrylamide gel electrophoresis based sulfotransferase assay

BACKGROUND: Sulfotransferases are a large group of enzymes that regulate the biological activity or availability of a wide spectrum of substrates through sulfation with the sulfur donor 3'-phosphoadenosine-5'-phosphosulfate (PAPS). These enzymes are known to be difficult to assay. A conven...

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Main Authors: Wu, Zhengliang L, Ethen, Cheryl M, Larson, Sara, Prather, Brittany, Jiang, Weiping
Format: Artigo
Jezik:Inglês
Izdano: BioMed Central 2010
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2834601/
https://ncbi.nlm.nih.gov/pubmed/20146816
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1472-6750-10-11
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