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Affinity Labeling of the Heavy and Light Chains of a Myeloma Protein with Anti-2,4-Dinitrophenyl Activity

A mouse myeloma protein with high affinity for 2,4-dinitrophenyl (Dnp) ligands was reacted with the bromoacetyl derivatives of N-Dnp-ethylenediamine and (ε)-N-Dnp-L-lysine. Up to 1.4 sites per protein molecule were covalently labeled. The labeling reactions were essentially completely blocked by a l...

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Veröffentlicht in:Proc Natl Acad Sci U S A
Hauptverfasser: Haimovich, Joseph, Givol, David, Eisen, Herman N.
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 1970
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Online Zugang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC283408/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/4099105/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.67.4.1656
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