A mutant RNA polymerase that forms unusual open promoter complexes

We describe a mutant Escherichia coli RNA polymerase (RNAP) that forms stable open promoter complexes even at −20°C but with a shortened melted region that extends downstream to only position −7. In the presence of initiating transcription substrates, the mutant RNAP undergoes a temperature-dependen...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	Severinov, Konstantin, Darst, Seth A.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 1997
Soggetti:	Biological Sciences
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC28331/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9391051/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.94.25.13481
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