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Isolation and purification of Thermus thermophilus HpaB by a crystallization approach

The oxygenase HpaB is a component of the 4-hydroxyphenylacetate 3-monooxygenase enzyme that is responsible for the hydroxylation of 4-hydroxy­phenylacetate. It utilizes molecular oxygen and a reduced flavin, which is provided by HpaC, the second component of the enzyme. While isolating integral memb...

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Bibliografiske detaljer
Main Authors: Soulimane, Tewfik, O’Kane, Sarah R., Kolaj, Olga
Format: Artigo
Sprog:Inglês
Udgivet: International Union of Crystallography 2010
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2833055/
https://ncbi.nlm.nih.gov/pubmed/20208179
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309110003714
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