Generation of Backbone-Free, Low Transgene Copy Plants by Launching T-DNA from the Agrobacterium Chromosome(1)([W])([OA])

In both applied and basic research, Agrobacterium-mediated transformation is commonly used to introduce genes into plants. We investigated the effect of three Agrobacterium tumefaciens strains and five transferred (T)-DNA origins of replication on transformation frequency, transgene copy number, and...

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Autores principales: Oltmanns, Heiko, Frame, Bronwyn, Lee, Lan-Ying, Johnson, Susan, Li, Bo, Wang, Kan, Gelvin, Stanton B.
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society of Plant Biologists 2010
Materias:
Breakthrough Technologies
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2832237/
https://ncbi.nlm.nih.gov/pubmed/20023148
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1104/pp.109.148585
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