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Partial Purification of the Ouabain-Binding Component and of Na,K-ATPase from Human Red Cell Membranes

Ghosts of human red cells were incubated with tritiated ouabain in the presence of ATP, Mg, and Na, conditions under which ouabain binds with high specificity to Na:K transport sites. The labeled membranes were solubilized with sodium dodecyl sulfate and dialyzed. Sodium dodecyl sulfate solubilizes...

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Detalles Bibliográficos
Autores principales: Dunham, Philip B., Hoffman, Joseph F.
Formato: Artigo
Lenguaje:Inglês
Publicado: 1970
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC283141/
https://ncbi.nlm.nih.gov/pubmed/4246615
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