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Preparation of stable isotope-labeled peripheral cannabinoid receptor CB2 by bacterial fermentation

We developed a bacterial fermentation protocol for production of a stable isotope-labeled cannabinoid receptor CB2 for subsequent structural studies of this protein by nuclear magnetic resonance spectroscopy. The human peripheral cannabinoid receptor was expressed in Escherichia coli as a fusion wit...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Berger, Christian, Ho, Jenny T.C., Kimura, Tomohiro, Hess, Sonja, Gawrisch, Klaus, Yeliseev, Alexei
Format: Artigo
Sprache:Inglês
Veröffentlicht: 2010
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2827653/
https://ncbi.nlm.nih.gov/pubmed/20044006
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.pep.2009.12.011
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