In Vitro Bioassay of Endotoxin Using Fluorescein as a pH Indicator in a Macrophage Cell Culture System

Based on the biological activity of endotoxin, we propose a possible new method for detecting endotoxin using a pH-indication system of macrophage culture media. After RAW 264.7 macrophage cells were treated with lipopolysaccharide (LPS), the addition of fluorescein to the LPS-treated media reproduc...

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Main Authors: Lee, Dong Hee, Sung, Hak-Joon, Han, Dong-Wook, Lee, Min-Sub, Ryu, Gyu Ha, Aihara, Maki, Takatori, Kosuke, Park, Jong-Chul
Formato: Artigo
Idioma:Inglês
Publicado: Yonsei University College of Medicine 2005
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Original Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2823024/
https://ncbi.nlm.nih.gov/pubmed/15861501
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3349/ymj.2005.46.2.268
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