Simple and effective purification of a Na+-dependent amino acid transport system from Ehrlich ascites cell plasma membrane.
A reconstitution assay was used to measure transport activity during purification of a Na+-dependent amino acid transporter from Ehrlich cell plasma membrane. Cholate/urea-solubilized membranes were fractionated on a Sepharose 6B column and transport activity was recovered in the column void volume....
Guardat en:
| Publicat a: | Proc Natl Acad Sci U S A |
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| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
National Academy of Sciences
1988
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC282300/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2847146/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.21.7877 |
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