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Mining mammalian genomes for folding competent proteins using Tat-dependent genetic selection in Escherichia coli
Recombinant expression of eukaryotic proteins in Escherichia coli is often limited by poor folding and solubility. To address this problem, we employed a recently developed genetic selection for protein folding and solubility based on the bacterial twin-arginine translocation (Tat) pathway to rapidl...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Wiley Subscription Services, Inc., A Wiley Company
2009
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2821272/ https://ncbi.nlm.nih.gov/pubmed/19830686 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.262 |
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