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Thermococcus kodakarensis Genetics: TK1827-Encoded β-Glycosidase, New Positive-Selection Protocol, and Targeted and Repetitive Deletion Technology

Inactivation of TK1761, the reporter gene established for Thermococcus kodakarensis, revealed the presence of a second β-glycosidase that we have identified as the product of TK1827. This enzyme (pTK1827) has been purified and shown to hydrolyze glucopyranoside but not mannopyranoside, have optimal...

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Détails bibliographiques
Auteurs principaux: Santangelo, Thomas J., Čuboňová, L'ubomíra, Reeve, John N.
Format: Artigo
Langue:Inglês
Publié: American Society for Microbiology (ASM) 2010
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Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2820962/
https://ncbi.nlm.nih.gov/pubmed/20023088
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02497-09
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