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Detection of DNA looping due to simultaneous interaction of a DNA-binding protein with two spatially separated binding sites on DNA.

We describe different and relatively rapid biochemical techniques to detect protein-mediated DNA looping. These techniques, based on enhancement of DNA knotting and that of ligase-catalyzed cyclization, were used to show that the replication initiator protein of plasmid R6K can bring together two in...

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Udgivet i:Proc Natl Acad Sci U S A
Principais autores: Mukherjee, S, Erickson, H, Bastia, D
Format: Artigo
Sprog:Inglês
Udgivet: National Academy of Sciences 1988
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC281954/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3413096/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.17.6287
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