Exploring the Roles of the Metal Ions in Escherichia coli Copper Amine Oxidase(,)

[Image: see text] To investigate the role of the active site copper in Escherichia coli copper amine oxidase (ECAO), we initiated a metal-substitution study. Copper reconstitution of ECAO (Cu-ECAO) restored only ∼12% wild-type activity as measured by k(cat(amine)). Treatment with EDTA, to remove exo...

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Autori principali: Smith, Mark A., Pirrat, Pascale, Pearson, Arwen R., Kurtis, Christian R. P., Trinh, Chi H., Gaule, Thembaninkosi G., Knowles, Peter F., Phillips, Simon E. V., McPherson, Michael J.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Chemical Society 2010
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2817917/
https://ncbi.nlm.nih.gov/pubmed/20052994
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi901738k
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