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Mutational analysis of active-site residues in the Mycobacterium leprae RecA intein, a LAGLIDADG homing endonuclease: Asp(122) and Asp(193) are crucial to the double-stranded DNA cleavage activity whereas Asp(218) is not

Mycobacterium leprae recA harbors an in-frame insertion sequence that encodes an intein homing endonuclease (PI-MleI). Most inteins (intein endonucleases) possess two conserved LAGLIDADG (DOD) motifs at their active center. A common feature of LAGLIDADG-type homing endonucleases is that they recogni...

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Bibliografske podrobnosti
Main Authors: Singh, Pawan, Tripathi, Pankaj, Muniyappa, K
Format: Artigo
Jezik:Inglês
Izdano: Wiley Subscription Services, Inc., A Wiley Company 2010
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2817846/
https://ncbi.nlm.nih.gov/pubmed/19937653
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.292
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