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Cloning, expression, purification, crystallization and preliminary X-ray diffraction analysis of the collagen-binding region of RspB from Erysipelothrix rhusiopathiae

RspB is a surface adhesin of Erysipelothrix rhusiopathiae. A recombinant form of the collagen-binding region of this protein, RspB((31–348)), has been overexpressed in Escherichia coli in native and selenomethionine-derivative forms and purified using affinity and gel-permeation chromatography. Thin...

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Main Authors: Devi, Aribam Swarmistha, Ogawa, Yohsuke, Shimoji, Yoshihiro, Ponnuraj, Karthe
Format: Artigo
Jezik:Inglês
Izdano: International Union of Crystallography 2010
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2815681/
https://ncbi.nlm.nih.gov/pubmed/20124711
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309109035581
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