Performance of serum-supplemented and serum-free media in IFNγ Elispot Assays for human T cells

The choice of serum for supplementation of media for T cell assays and in particular, Elispot has been a major challenge for assay performance, standardization, optimization, and reproducibility. The Assay Working Group of the Cancer Vaccine Consortium (CVC-CRI) has recently identified the choice of...

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Dettagli Bibliografici
Autori principali: Janetzki, Sylvia, Price, L., Britten, C. M., van der Burg, S. H., Caterini, J., Currier, J. R., Ferrari, G., Gouttefangeas, C., Hayes, P., Kaempgen, E., Lennerz, V., Nihlmark, K., Souza, V., Hoos, A.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Springer-Verlag 2009
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Original Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2813531/
https://ncbi.nlm.nih.gov/pubmed/19894047
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s00262-009-0788-2
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