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Single Molecule- and Fluorescence Correlation Spectroscopy-FRET Analysis of Phage DNA Packaging: Co-localization of the Packaged Phage T4 DNA Ends within the Capsid
Linear DNAs of any sequence can be packaged with high efficiency in vitro into empty viral procapsids by the phage T4 terminase. Packaging substrates of 5 and 50 kbps, terminated by energy transfer dye pairs, were constructed from plasmid and lambda phage DNAs. Nuclease and fluorescence correlation...
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| Auteurs principaux: | , , , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
2009
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2813382/ https://ncbi.nlm.nih.gov/pubmed/19962991 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2009.11.067 |
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