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Molecular Detection of Viable Bacterial Pathogens in Water by Ratiometric Pre-rRNA Analysis

Ratiometric pre-rRNA analysis (RPA) detects the replenishment of rRNA precursors that occurs rapidly upon nutritional stimulation of bacterial cells. In contrast to DNA detection by PCR, RPA distinguishes viable from inactivated bacteria. It exhibits promise as a molecular viability test for pathoge...

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Hlavní autoři: Cangelosi, Gerard A., Weigel, Kris M., Lefthand-Begay, Clarita, Meschke, John S.
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology (ASM) 2010
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2812999/
https://ncbi.nlm.nih.gov/pubmed/19948855
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01810-09
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