Development of a Loop-Mediated Isothermal Amplification Assay for Sensitive and Rapid Detection of the tdh and trh Genes of Vibrio parahaemolyticus and Related Vibrio Species

Thermostable direct hemolysin (TDH) and TDH-related hemolysin (TRH) are the major virulence determinants of Vibrio parahaemolyticus. TRH is further differentiated into TRH1 and TRH2 on the basis of genetic and phenotypic differences. We developed a novel and highly specific loop-mediated isothermal...

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Main Authors: Yamazaki, Wataru, Kumeda, Yuko, Misawa, Naoaki, Nakaguchi, Yoshitsugu, Nishibuchi, Mitsuaki
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2010
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Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2812988/
https://ncbi.nlm.nih.gov/pubmed/19966027
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02284-09
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