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Construction of a laser combiner for dual fluorescent single molecule imaging of pRNA of phi29 DNA packaging motor

A customized laser combiner was designed and constructed for dual channel single molecule imaging. The feasibility of a combiner-incorporated imaging system was demonstrated in studies of single molecule FRET. Distance rulers made of dual-labeled dsDNA were used to evaluate the system by determining...

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Detalhes bibliográficos
Main Authors: Zhang, Hui, Shu, Dan, Browne, Mark, Guo, Peixuan
Formato: Artigo
Idioma:Inglês
Publicado em: Springer US 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2812712/
https://ncbi.nlm.nih.gov/pubmed/19809878
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/s10544-009-9364-y
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