Selection of reference genes for quantitative RT-PCR studies in Rhipicephalus (Boophilus) microplus and Rhipicephalus appendiculatus ticks and determination of the expression profile of Bm86

BACKGROUND: For accurate and reliable gene expression analysis, normalization of gene expression data against reference genes is essential. In most studies on ticks where (semi-)quantitative RT-PCR is employed, normalization occurs with a single reference gene, usually β-actin, without validation of...

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Autores principales: Nijhof, Ard M, Balk, Jesper A, Postigo, Milagros, Jongejan, Frans
Formato: Artigo
Lenguaje:Inglês
Publicado: BioMed Central 2009
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Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2809063/
https://ncbi.nlm.nih.gov/pubmed/20040102
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2199-10-112
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