Enrichment and Site Mapping of O-Linked N-Acetylglucosamine by a Combination of Chemical/Enzymatic Tagging, Photochemical Cleavage, and Electron Transfer Dissociation Mass Spectrometry

Numerous cellular processes are regulated by the reversible addition of either phosphate or O-linked β-N-acetylglucosamine (O-GlcNAc) to nuclear and cytoplasmic proteins. Although sensitive methods exist for the enrichment and identification of protein phosphorylation sites, those for the enrichment...

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Bibliografiske detaljer
Main Authors: Wang, Zihao, Udeshi, Namrata D., O'Malley, Meaghan, Shabanowitz, Jeffrey, Hunt, Donald F., Hart, Gerald W.
Format: Artigo
Sprog:Inglês
Udgivet: The American Society for Biochemistry and Molecular Biology 2010
Fag:
Research
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2808261/
https://ncbi.nlm.nih.gov/pubmed/19692427
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/mcp.M900268-MCP200
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