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On-plate digestion using a commercial micro-fraction collector for nano-HPLC MALDI-TOF/TOF protein analysis

A new method for on-plate protein digestion and MALDI MS analysis is proposed involving an automated one-step sample separation using nanoflow-HPLC followed by nanoliter fraction collection and on-plate digestion with trypsin. This procedure uses a commercial automatic nanoliter fraction collection...

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Detalhes bibliográficos
Main Authors: Vitorino, Rui, Guedes, Sofia, Tomer, Kenneth, Domingues, Pedro, Duarte, José, Amado, Francisco
Formato: Artigo
Idioma:Inglês
Publicado em: 2008
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2805255/
https://ncbi.nlm.nih.gov/pubmed/18519023
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2008.05.006
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