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Deep Sequencing of a Dimethylsulfoniopropionate-Degrading Gene (dmdA) by Using PCR Primer Pairs Designed on the Basis of Marine Metagenomic Data

In silico design and testing of environmental primer pairs with metagenomic data are beneficial for capturing a greater proportion of the natural sequence heterogeneity in microbial functional genes, as well as for understanding limitations of existing primer sets that were designed from more restri...

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Hlavní autoři: Varaljay, Vanessa A., Howard, Erinn C., Sun, Shulei, Moran, Mary Ann
Médium: Artigo
Jazyk:Inglês
Vydáno: American Society for Microbiology (ASM) 2010
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2805212/
https://ncbi.nlm.nih.gov/pubmed/19948858
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01258-09
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