High-throughput isolation of immunoglobulin genes from single human B cells and expression as monoclonal antibodies

Defining human B cell repertoires to viral pathogens is critical for design of vaccines that induce broadly protective antibodies to infections such as HIV-1 and influenza. Single B cell sorting and cloning of immunoglobulin (Ig) heavy- and light-chain variable regions (V(H) and V(L)) is a powerful...

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Main Authors: Liao, Hua-Xin, Levesque, Marc C., Nagel, Ashleigh, Dixon, Ashlyn, Zhang, Ruijun, Walter, Emmanuel, Parks, Robert, Whitesides, John, Marshall, Dawn J., Hwang, Kwan-Ki, Yang, Yi, Chen, Xi, Gao, Feng, Munshaw, Supriya, Kepler, Thomas B., Denny, Thomas, Moody, M. Anthony, Haynes, Barton F.
格式: Artigo
語言:Inglês
出版: 2009
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Article
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC2805188/
https://ncbi.nlm.nih.gov/pubmed/19428587
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2009.02.014
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