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Precise detection of pH inside large unilamellar vesicles using membrane-impermeable dendritic porphyrin-based nanoprobes

Accurate real-time measurements of proton concentration gradients are pivotal to mechanistic studies of proton translocation by membrane-bound enzymes. Here we report a detailed characterization of the pH-sensitive fluorescent nanoprobe Glu(3), which is well suited for pH measurements in microcompar...

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Detalhes bibliográficos
Main Authors: Leiding, Thom, Górecki, Kamil, Kjellman, Tomas, Vinogradov, Sergei A., Hägerhäll, Cecilia, Årsköld, Sindra Peterson
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2804896/
https://ncbi.nlm.nih.gov/pubmed/19248752
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ab.2009.02.023
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