Characterization of a zinc blotting technique: evidence that a retroviral gag protein binds zinc.
We have characterized a simple method that uses 65ZnCl2 to detect zinc-binding proteins that have been immobilized on nitrocellulose. Conditions have been identified that permit the detection of as little as 1 microgram of some zinc-binding proteins. The specificity of the binding is indicated by th...
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| Publié dans: | Proc Natl Acad Sci U S A |
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| Auteurs principaux: | , , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
National Academy of Sciences
1988
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC280393/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3260031/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.12.4195 |
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