Tertiary Structure and Characterization of a Glycoside Hydrolase Family 44 Endoglucanase from Clostridium acetobutylicum

A gene encoding a glycoside hydrolase family 44 (GH44) protein from Clostridium acetobutylicum ATCC 824 was synthesized and transformed into Escherichia coli. The previously uncharacterized protein was expressed with a C-terminal His tag and purified by nickel-nitrilotriacetic acid affinity chromato...

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Autors principals: Warner, Christopher D., Hoy, Julie A., Shilling, Taran C., Linnen, Michael J., Ginder, Nathaniel D., Ford, Clark F., Honzatko, Richard B., Reilly, Peter J.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology (ASM) 2010
Matèries:
Enzymology and Protein Engineering
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2798630/
https://ncbi.nlm.nih.gov/pubmed/19915043
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02026-09
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