Phenol emulsion-enhanced DNA-driven subtractive cDNA cloning: isolation of low-abundance monkey cortex-specific mRNAs.

To isolate cDNA clones of low-abundance mRNAs expressed in monkey cerebral cortex but absent from cerebellum, we developed an improved subtractive cDNA cloning procedure that requires only modest quantities of mRNA. Plasmid DNA from a monkey cerebellum cDNA library was hybridized in large excess to...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	Travis, G H, Sutcliffe, J G
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 1988
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC279841/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2894033/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.5.1696
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