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Characterization of Mouse UDP-glucose Pyrosphosphatase, a Nudix Hydrolase encoded by the Nudt14 Gene
Recombinant mouse UDP-glucose pyrophosphatase (UGPPase), encoded by the Nudt14 gene, was produced in Escherichia coli and purified close to homogeneity. The enzyme catalyzed the conversion of [β-(32)P]UDP-glucose to (32)P-glucose-1-P and UMP, confirming that it hydrolyzed the pyrophosphate of the nu...
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| Autors principals: | , , , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2009
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2798163/ https://ncbi.nlm.nih.gov/pubmed/19896456 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bbrc.2009.11.007 |
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