Optimized PCR with sequence specific primers (PCR-SSP) for fast and efficient determination of Interleukin-6 Promoter (-597/-572/-174)Haplotypes

BACKGROUND: Interleukin-6 (IL-6) promoter polymorphisms at positions -597(G→A), -572(G→C) and -174(G→C) were shown to have a clinical impact on different major diseases. At present PCR-SSP protocols for IL-6 (-597/-572/-174)haplotyping are elaborate and require large amounts of genomic DNA. FINDINGS...

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Detalles Bibliográficos
Main Authors: Müller-Steinhardt, Michael, Schulte, Friederike, Klüter, Harald, Bugert, Peter
Formato: Artigo
Idioma:Inglês
Publicado: BioMed Central 2009
Assuntos:
Short Report
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2797812/
https://ncbi.nlm.nih.gov/pubmed/20003261
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-2-245
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