Expression of soluble, enzymatically active, human immunodeficiency virus reverse transcriptase in Escherichia coli and analysis of mutants.

We have constructed a plasmid that, when introduced into Escherichia coli, induces the synthesis of large quantities of a protein with an apparent molecular mass of 66 kDa that differs from human immunodeficiency virus (HIV) RNA-dependent DNA polymerase (deoxynucleoside-triphosphate:DNA deoxynucleot...

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Bibliographic Details
Published in:Proc Natl Acad Sci U S A
Main Authors: Hizi, A, McGill, C, Hughes, S H
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 1988
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC279738/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2448794/
https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.4.1218
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