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Functional analysis of the four DNA binding domains of Replication Protein A: the role of RPA2 in ssDNA binding

Replication Protein A (RPA), the heterotrimeric SSB of eukaryotes, contains four ssDNA binding domains (DBDs) within its two largest subunits, RPA1 and RPA2. We analyzed the contribution of the four DBDs to ssDNA binding affinity by assaying recombinant heterotrimeric RPA in which a single DBD (A, B...

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Autori principali: Bastin-Shanower, Suzanne A., Brill, Steven J.
Natura: Artigo
Lingua:Inglês
Pubblicazione: 2001
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC2796477/
https://ncbi.nlm.nih.gov/pubmed/11479296
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M104386200
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