Simultaneous transfection of COS-1 cells with mitochondrial and microsomal steroid hydroxylases: incorporation of a steroidogenic pathway into nonsteroidogenic cells.

Transfected, nonsteroidogenic COS-1 cells derived from monkey kidney are found to be capable of supporting the initial and rate-limiting step common to all steroidogenic pathways, the side-chain cleavage of cholesterol to produce pregnenolone. Endogenous COS-1 kidney cell renodoxin reductase and ren...

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Pubblicato in:	Proc Natl Acad Sci U S A
Autori principali:	Zuber, M X, Mason, J I, Simpson, E R, Waterman, M R
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	National Academy of Sciences 1988
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC279622/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2829199/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.85.3.699
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