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Purification, crystallization and preliminary crystallographic analysis of the [NiFeSe] hydrogenase from Desulfovibrio vulgaris Hildenborough

The [NiFeSe] hydrogenases belong to a subgroup of the [NiFe] proteins in which a selenocysteine is a ligand of the Ni. These enzymes demonstrate interesting catalytic properties, showing a very high H(2)-producing activity that is sustained in the presence of low O(2) concentrations. The purificatio...

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Detalhes bibliográficos
Main Authors: Marques, Marta, Coelho, Ricardo, Pereira, Inês A. C., Matias, Pedro M.
Formato: Artigo
Idioma:Inglês
Publicado em: International Union of Crystallography 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2795601/
https://ncbi.nlm.nih.gov/pubmed/19724133
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309109031261
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