Optimization of a Rapid Viability Assay for Mycobacterium avium subsp. paratuberculosis by Using alamarBlue

A microtiter alamarBlue assay was adapted and optimized for Mycobacterium avium subsp. paratuberculosis. Using cell concentrations ranging from 10(4) to 10(8) CFU/ml, a minimum incubation time to indicate viability was obtained after 24 h. Rifampin (rifampicin) was used to demonstrate that this meth...

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Bibliografiska uppgifter
Huvudupphovsmän: Carroll, James, Douarre, Pierre, Coffey, Aidan, Buckley, Jim, Cashman, Bill, O'Farrell, Kevin, O'Mahony, Jim
Materialtyp: Artigo
Språk:Inglês
Publicerad: American Society for Microbiology (ASM) 2009
Ämnen:
Methods
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC2794114/
https://ncbi.nlm.nih.gov/pubmed/19837835
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01203-09
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