Simultaneous assay of every Salmonella Typhi gene using one million transposon mutants

Very high-throughput sequencing technologies need to be matched by high-throughput functional studies if we are to make full use of the current explosion in genome sequences. We have generated a very large bacterial mutant pool, consisting of an estimated 1.1 million transposon mutants and we have u...

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Autors principals: Langridge, Gemma C., Phan, Minh-Duy, Turner, Daniel J., Perkins, Timothy T., Parts, Leopold, Haase, Jana, Charles, Ian, Maskell, Duncan J., Peters, Sarah E., Dougan, Gordon, Wain, John, Parkhill, Julian, Turner, A. Keith
Format: Artigo
Idioma:Inglês
Publicat: Cold Spring Harbor Laboratory Press 2009
Matèries:
Methods
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2792183/
https://ncbi.nlm.nih.gov/pubmed/19826075
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.097097.109
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