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siDirect 2.0: updated software for designing functional siRNA with reduced seed-dependent off-target effect
BACKGROUND: RNA interference (RNAi), mediated by 21-nucleotide (nt)-length small interfering RNAs (siRNAs), is a powerful tool not only for studying gene function but also for therapeutic applications. RNAi, requiring perfect complementarity between the siRNA guide strand and the target mRNA, was be...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2791777/ https://ncbi.nlm.nih.gov/pubmed/19948054 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2105-10-392 |
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