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Bacterial display enables efficient and quantitative peptide affinity maturation

A quantitative screening method was developed to enable isolation and affinity maturation of peptide ligands specific for a given target from peptide libraries displayed on the outer surface of Escherichia coli using multi-parameter flow cytometry. From a large, random 15-mer peptide library, screen...

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Autores principales: Kenrick, Sophia A., Daugherty, Patrick S.
Formato: Artigo
Lenguaje:Inglês
Publicado: Oxford University Press 2010
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2791049/
https://ncbi.nlm.nih.gov/pubmed/19903738
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/protein/gzp065
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