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Bacterial display enables efficient and quantitative peptide affinity maturation
A quantitative screening method was developed to enable isolation and affinity maturation of peptide ligands specific for a given target from peptide libraries displayed on the outer surface of Escherichia coli using multi-parameter flow cytometry. From a large, random 15-mer peptide library, screen...
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| Main Authors: | , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
Oxford University Press
2010
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2791049/ https://ncbi.nlm.nih.gov/pubmed/19903738 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/protein/gzp065 |
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