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Quantitative assessment of enzyme specificity in vivo: P(2) recognition by Kex2 protease defined in a genetic system

The specificity of the yeast proprotein-processing Kex2 protease was examined in vivo by using a sensitive, quantitative assay. A truncated prepro-α-factor gene encoding an α-factor precursor with a single α-factor repeat was constructed with restriction sites for cassette mutagenesis flanking the s...

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Autors principals: Bevan, Alison, Brenner, Charles, Fuller, Robert S.
Format: Artigo
Idioma:Inglês
Publicat: The National Academy of Sciences 1998
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC27903/
https://ncbi.nlm.nih.gov/pubmed/9724712
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